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KMID : 0438619940180010063
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Journal of Oral Biology
1994 Volume.18 No. 1 p.63 ~ p.70
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Influences of Cholecystokinin on Potassium Channels in Submaxillary Acinar Cells of Rat
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Kim, Kyung-Nyun
Kim, Joong-Soo/Lee, Jong-Heun
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Abstract
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The saliva is formed by outflow of potassium due to increase of intracellular calcium activity. The principal passage of potassium ions is the calcium-activated potassium channels. This study was performed to elucidate the change in the activity of calcium-activated potassium channels during salivary secretion induced by acetylcholine (ACh) or cholecystokinin(CCK). The submaxillary acinar cells were collected from Sprague-Dawley rat (about 200 g body weight) by time-scheduled trypsin and collagenase treatment. Dispersed acinar cells were placed in 100% oxygenated HEPES-buffered Tyrode solution and perfused. Cell attached or excised inside out patch clamp method was employed. The channel activities recorded via mocropipettes (1-5 ㏁ resistance) filled with HEPES-buffered high potassium solution were evaluated with personal computer. Two distinct types of potassium channels in membranes of submaxillary acinar cells were observed. The one type had large conductance and was sensitive to pipette voltage and calcium ion activity, but the other type had small conductance and was not sensitive to pipette voltage. Addition of ACh and/or CCK increased the open probability of potassium channels without change in conductance. These results suggest that CCK could evoke salivary secretion via the same mechanism as ACh.
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